FIG. 6.

Effect of conditioned media harvested from WT (HO-1^+/+) or KO (HO-1^−/−) BMDCs on blood flow recovery in hyperglycemic HET (HO-1^+/−) muscle. Media (30 μl in total) were injected into three sites of ischemic caput gastrocnemius muscle of mice subjected to femoral artery ligation. Injections were performed immediately after surgery (day 0), on day 1, and on day 2. Unconditioned media served as a control. (A, B) Blood flow recovery in ischemic muscle. Laser Doppler analysis. Quantitative data (A) and representative scans (B). (C) Percentage of necrotic toes. *p<0.05, **p<0.01, and ***p<0.001 versus control; ^#p<0.05, ^##p<0.01, and ^###p<0.001 versus day 0. To see this illustration in color, the reader is referred to the web version of this article at www.liebertpub.com/ars