FIG. 8.

Lack of Nrf2 changes its target gene expression and antioxidant capacity in ischemic muscles after FAL. Nrf2^+/+ and Nrf2^−/− mice were subjected to FAL. (A–C) Preoperatively (untreated) and at days 1 and 3 after surgery the mRNA level of, respectively, Nrf2, HO-1, and NQO1 was checked in caput gastrocnemius. RT-PCR (n_Nrf2+/+=10–12, n_Nrf2−/−=10–12). (D) Total antioxidant capacity (copper reducing equivalents [CREs]) is lower in Nrf2^−/− ischemic muscles (n_Nrf2+/+=4, n_Nrf2−/−=4); each bar represents the mean±SEM. *p<0.05, untreated versus ischemia (appropriate time point); ^#p<0.05, Nrf2^+/+ versus Nrf2^−/−.