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. 2014 Mar 21;20(11):2913–2926. doi: 10.3748/wjg.v20.i11.2913

Figure 2.

Figure 2

Analysis of protein expression following knockdown of endogenous DDX5, DDX3 and DDX6 in Huh7.5 cells. A: Cells transfected with 40 nmol/L of DDX5 short interfering RNA (siRNA) or a control siRNA; B: Cells transfected with 40 nmol/L of DDX3 siRNA or control siRNA; C: Cells transfected with 40 nmol/L of DDX6 siRNA or control siRNA. At 48 h, cells were infected with J6/JFH-1 (p47) hepatitis C virus (HCV) at multiplicity of infection of 2 as previously described[72]. Cell lysates were prepared 0, 2, 4 d post-infection (d.p.i.) and subjected to immunoblotting with the respective primary antibodies. Expression of NS5B was used to confirm HCV infection and β-actin served as a loading control. Three independent experiments were performed and one representative set of data is shown.