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Quantitation of hepatic transformation growth factor-β mRNA expression level using real-time reverse-transcriptase polymerase chain reaction analysis. Results are mean values of 2 independent experiments with 10 rats in each group. The mean value of the normalized hepatic transformation growth factor-β (TGF-β)/β-actin mRNA expression ratio in the hyperoxic rats was significantly higher than that in the untreated rats. aP < 0.05 vs the corresponding values in the control group.
