Clonogenic survival of Gli36 cells. Cells were exposed to radiation doses of 0–8 Gy, with and without 1 h pretreatment with Pluronic L10 (0.1 and 0.3 mg/ml). After 10 days incubation, colonies were fixed and stained, and colonies containing > 50 cells were counted. Surviving fraction was determined by dividing the plating efficiency of irradiated cultures by the plating efficiency of un-irradiated cultures, which was 70.8% without L10 and 65.9% and 61.9% for 0.1 and 0.3 mg/ml L10, respectively. Survival curve data from three independent experiments are expressed as mean with ± SD. * The difference between radiation and L10 + radiation values were significant at each dose (p < 0.05).