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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Int J Radiat Biol. 2013 Jun 3;89(10):801–812. doi: 10.3109/09553002.2013.800246

Figure 5.

Figure 5

Flow cytometric detection of γ-H2AX formation in Gli36 cells after irradiating with Pluronic L10. (A) The bivariate distribution of cells 2 h after treatment with 0.3 mg/ml Pluronic L10, 3 Gy, or Pluronic L10 + 3 Gy. Y axis is γ-H2AX staining and X axis is the DNA content. Notice the presence of increased γ-H2AX staining (as gated) after combined treatment. (B) Percentage of cells with γ-H2AX staining after irradiation (3 Gy) with and without pretreatment with 0.3 mg/ml Pluronic L10. Analysis was carried out by flow cytometry after γ-H2AX staining of treated cells. Two hours after treatment, cells treated with both Pluronic L10 and radiation show the highest percentage of γ-H2AX staining. Values are an average of three independent experiments ± SD.