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. Author manuscript; available in PMC: 2014 Mar 21.
Published in final edited form as: J Neurochem. 2012 Aug 23;123(2):288–297. doi: 10.1111/j.1471-4159.2012.07875.x

Figure 3. Distribution of DAT within striatal synaptosomal membrane and vesicle fractions.

Figure 3

(A) Synaptosomal membrane and total vesicle fractions were isolated from striatal synaptosomes, as described in Materials & Methods, and DAT immunoreactivity was measured by densitometry (n = 3 per group). (B) Distribution of DAT within synaptosomal vesicle fractions isolated across 0.6M – 1.6M continuous sucrose gradients and the synaptosomal membrane fraction. The recycling endosome marker rab11 and lysosome marker LAMP2 localized to DAT within the vesicle fractions, whereas the plasma membrane protein Na+/K+ ATPase did not. ’SM’ indicates synaptosomal membrane fraction.