Rats received either single or multiple injections of saline (single = 1 ml/kg, s.c.; multiple = 4 × 1 ml/kg/injection, 2-h intervals, s.c.) or METH (single = 15 mg/kg, s.c.; multiple = 4 × 7.5 mg/kg/injection, 2-h intervals, s.c.) and sacrificed 60 min following treatment (n = 6 per group). No changes in DAT immunoreactivity within the synaptosomal membrane (A, C) or vesicle (B, D) fractions were observed with either treatment paradigm. ‘DAT P’ indicates DAT peptide control lane.