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. Author manuscript; available in PMC: 2014 Mar 21.
Published in final edited form as: Neuron. 2006 Oct 19;52(2):255–269. doi: 10.1016/j.neuron.2006.09.037

Figure 2. Characterization of S421 as a Site of Activity-Dependent Phosphorylation on MeCP2.

Figure 2

(A–C) Kinetics of MeCP2 S421 phosphorylation and dephosphorylation. Lysates were prepared from rat E18 + 5 DIV cortical neurons membrane depolarized for the indicated times (A and B) or membrane depolarized for 30 min and repolarized with culture medium for the indicated times (C).

(D–F) MeCP2 is phosphorylated at S421 in response to glutamate (D), NMDA (E), and bicuculline (F) in a calcium-dependent manner. Antitotal MeCP2 and anti-MeCP2 pS421 western blots of extracts from E18 + 12 DIV hippocampal neurons (D and E) or P1 + 12 DIV hippocampal neurons (F) treated for 1 hr with the indicated stimuli after a 1 hr pretreatment with the indicated blockers. Nimo, nimodipine.

(G) MeCP2 S421 phosphorylation is triggered by neurotrophins. Western blot analysis of whole-cell extracts prepared from E18 + 12 DIV hippocampal neurons stimulated for 1 hr with BDNF, CNTF, EGF, IGF, NGF, NT3, NT4, or PDGF.