Skip to main content
. Author manuscript; available in PMC: 2014 Mar 21.
Published in final edited form as: Neuron. 2006 Oct 19;52(2):255–269. doi: 10.1016/j.neuron.2006.09.037

Figure 3. Phosphorylation of MeCP2 at S421 Is Triggered by Synaptic Activity in the Intact Brain.

Figure 3

(A) Whole-cell lysates were prepared from P19 rat brains 0, 10, or 30 min after metrazole-based seizure induction and incubated with antibodies specific to MeCP2 pS421, total MeCP2, pCREB (S133), or total CREB.

(B) Fluorescent immunohistochemistry of coronal cryosections from mouse parietal cortex collected from wild-type (WT) or Mecp2−/y (KO) mice 3 hr after kainic acid-based seizure induction using antibodies specific for MeCP2 pS421 or total MeCP2. Sections were counterstained with the nuclear dye Hoechst 33342.

(C) Light exposure during subjective night induces the phosphorylation of MeCP2 S421 in the suprachiasmatic nucleus (SCN). Wild-type mice were entrained to a 12hr:12hr light-dark cycle and then transferred to constant darkness. Two days later, entrained mice were either maintained in darkness (DD) or exposed to light for 2, 4, or 6 hr (LP2, LP4, LP6). Adjacent cryosections were alternately immunostained with antibodies specific for MeCP2 pS421 or total MeCP2.