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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Immunogenetics. 2014 Jan 28;66(4):267–279. doi: 10.1007/s00251-014-0759-4

Fig. 8. Analyses of pigr and PIGRL transcripts after infection.

Fig. 8

RT-qPCR was employed to quantify the relative levels of pigr and PIGRL transcripts during the initial stages of infection. Adult zebrafish were infected with either (a-c) Streptococcus iniae or (d-f) Snakehead rhabdovirus or mock infected. Expression analyses was conducted from (a, d) intestine, (b, e) spleen and (c, f) kidney. RNA samples were evaluated from 2 h to 24 h after infection. Transcript levels were normalized to eef1a1l1 by the ΔΔCt method (Livak and Schmittgen 2001). Analyses of pigrl1.4 are excluded for SHRV infections as expression was not reliably detected.