Figure 2.

Biosynthetic pathways of two model reduced biochemicals, poly(3-hydroxybutyrate) and 1,3-propanediol. Biochemical steps that consume reducing equivalents are highlighted in blue. Abbreviations are as follows: CoA, coenzyme A; PhaA, 3-ketoacyl-CoA thiolase; PhaB, acetoacetyl-CoA reductase; PhaC, poly(3-hydroxyalkanoate) synthase; DhaB, glycerol dehydratase; DhaT, 1,3-propanediol oxidoreductase. In most of the E. coli recombinants described in the text, the phaBAC genes were obtained from Azotobacter sp. strain FA8 [70].