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. Author manuscript; available in PMC: 2014 Mar 21.
Published in final edited form as: Cell Rep. 2014 Feb 13;6(4):748–764. doi: 10.1016/j.celrep.2014.01.028

Figure 2. UPF1 Promotes the Decay of mRNAs Encoding Proliferation Inhibitors and Differentiation Factors.

Figure 2

(A) qPCR analysis of transcripts encoding the indicated classes of proteins in Upf1-depleted (siUpf1) P19 cells. The fold change of mRNA level is relative to that in control cells treated with a siRNA against luciferase (siLuc). mRNA normalization and statistical analysis were performed as in Figure 1.

(B) RNA decay of selected mRNAs in P19 cells transfected as indicated (see Figure S2 for analysis of more mRNAs). Transcripts levels were normalized to the level of Gapdh mRNA, which is relatively stable.