. 2012 Jul 1;1:e201207001. doi: 10.5936/csbj.201207001

Table 1.

Oligonucleotide primers pairs used for site-directed mutagenesis. Mutated codons are in bold.

Mutations	Primer sequence (5’ → 3’)
Ile116 → Ala	GCTACAACGCACCGTCCGCTGACGGCACGCAGACG
Ile116 → Ala	CGTCTGCGTGCCGTCAGCGGACGGTGCGTTGTAGC
Ile116 → Cys	GCTACAACGCACCGTCCTGCGACGGCACGCAGACG
Ile116 → Cys	CGTCTGCGTGCCGTCGCAGGACGGTGCGTTGTAGC
Ile116 → Gly	GCTACAACGCACCGTCCGGAGACGGCACGCAGACG
Ile116 → Gly	CGTCTGCGTGCCGTCTCCGGACGGTGCGTTGTAGC
Ile116 → Ser	GCTACAACGCACCGTCCTCCGACGGCACGCAGACG
Ile116 → Ser	CGTCTGCGTGCCGTCGGAGGACGGTGCGTTGTAGC
Ile116 → Thr	GCTACAACGCACCGTCCACGGACGGCACGCAGACG
Ile116 → Thr	CGTCTGCGTGCCGTCCGTGGACGGTGCGTTGTAGC