Table 1.
Ump1 species | Polydispersity index | Stokes radius (Å) | Calculated molecular mass (kDa) | Percentage of scattering volume in solution |
---|---|---|---|---|
Ump1 wild-type monomer | 0.3 | 18.1 | 13.5 | 15.1% |
20.9 | 18.9 | 17.4% | ||
24.2 | 26.6 | 16.4% | ||
Ump1 wild-type dimer | 0.3 | 24.2 | 26.6 | 15.5% |
28 | 37.4 | 17.4% | ||
32.5 | 53.1 | 16.1% | ||
Ump1-C115S monomer | 0.2 | 20.9 | 18.9 | 15.7% |
24.2 | 26.6 | 16.9% | ||
28.1 | 39 | 15.4% |