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. 2013 Feb 28;4:e201301007. doi: 10.5936/csbj.201301007

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© Commisso et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly cited.

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R3M carrot cell suspension extract analyzed by HPLC-DAD and HPLC-ESI-MS.

A: Absorbance (245–600 nm) expressed in Arbitrary Units (mAU) using a scale is set from a minimum level of absorbance (-10 mAU) shown in purple, to a maximum (+100 mAU) shown in red. B: Chromatogram at 320 nm. C: Chromatogram at 520 nm. The peaks were characterized by LC-MS as follows. AC1: Cyanidin hexosyl pentosyl hexoside and cyanidin hexosyl pentoside. AC2 + AC3: Cyanidin caffeoyl hexosyl hexosyl hexoside and cyanidin caffeoyl hexosyl pentosyl hexoside. AC4: Cyanidin sinapoyl hexosyl pentosyl hexoside. AC5: Cyanidin feruloyl hexosyl pentosyl hexoside. AC6: Cyanidin coumaoryl hexosyl pentosyl hexoside. HC1: Caffeoyl quinic acid. HC2: Caffeoyl methyl quinic acid. HC3: Dicaffeoyl quinic acid. HC4: Caffeoyl methyl quinic acid and Caffeoyl dimethyl quinic acid. HB1 and HB2: Unknown compounds with absorbance spectra similar to hydroxybenzoic acids. D: Quantification of the 11 major peaks detected by LC-DAD. Black and white columns represent cells cultivated in constant darkness and under constant illumination, respectively. The values are the average of three independent biological replicates. E: LC-ESI-MS analysis of cells cultivated under constant illumination. Several peaks, which are not detected by LC-DAD, are also present in the chromatogram.