Table 3.

Fixed cell results. The columns PACT and Manual Count show the fraction of objects tracked after/before post-processing in bright-field images. Co-identified shows pairs of cells that were co-identified in fluorescence and bright field post-processed data and their corresponding RMSD

	Brightfield cells tracked After/Before post processing		Co-identified in fluorescence
	PACT	Manual	Pairs	RMSD
NIH 3T3/flat Si	26 / 32=81%	35 / 42 =83%	22	2.8 µm
NIH 3T3/Si black	21 / 40=52%	40 / 48 =83%	14	4.3 µm