Table 2.

Examples of addressing enzyme inhibition

Enzyme	Inhibitor	Ki (µM)	Performance	Source
homoserine dehydrogenase (ThrA) in E. coli CRS-BuOH 31				[51]
wild-type	threonine	n/a	produced 50 mg/L 1-propanol, 30 mg/L 1-butanol
ThrAfbrBC		n/a	produced >150 mg/L 1-propanol, 100 mg/L 1-butanol
DHAP synthase (AroG) in E. coli K12 ∆tyrR				[52, 56]
wild-type	L-phenylalanine	n/a	produced 6 ± 1 mg/L L-tyrosine
D146N		n/a	produced 71 ± 9 mg/L L-tyrosine
chorismate mutase / prephenate dehydrogenase (TyrA) in E. coli K12 ∆tyrR				[52, 57]
wild-type	L-tyrosine	n/a	produced 6 ± 1 mg/L L-tyrosine
M53I, A354V		n/a	produced 86 ± 9 mg/L L-tyrosine
citrate synthase in ethanologenic E. coli KO11				[53, 58]
E. coli gltA	NADH	2.8	produced 28 g/L ethanol
B. subtilis citZ		n/a	produced 42 g/L ethanol
dihydrolipoamide dehydrogenase (LPD) subunit of PDH in E. coli W3110				[54, 55]
wild-type	NADH	1.00	produced 7.3 mM ethanol
G354K		10.0	produced 125.8 mM ethanol

n/a – not available