Fig. 2.

Distribution of SsBBX24 in organs of Solanum plants. (A) Immunodetection of the SsBBX24 protein using either serum raised against His tag-SsBBX24 or pre-immune serum. Lanes 1 and 3, purified recombinant His-tagged SsBBX24 protein produced in E. coli strain M15REP4; lanes 2 and 4, extracts from S. sogarandinum plants. M, molecular weight marker (Sigma). (B) Immunoblot analysis of SsBBX24 amount in vegetative and reproductive organs of 2-month-old S. tuberosum phytotron-grown plants. Lane 1, young leaves (including very young developing leaves and terminal shoots); lane 2, well-expanded leaves; lane 3, old leaves; lane 4, stems; lane 5, tubers; lane 6, rhizomes; lane 7, roots; lane 8, stamens; lane 9, pistils; lane 10, petals; lane 11, sepals.