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. Author manuscript; available in PMC: 2014 Mar 21.
Published in final edited form as: Stem Cells. 2009 Mar;27(3):533–542. doi: 10.1634/stemcells.2008-0596

Figure 1.

Figure 1

Design of high-content screen for compounds that increase OCT4-positive hESCs. (A): hESCs were dissociated and plated at low density onto mouse embryonic fibroblasts, and small molecule inhibitors were added in duplicate 384-well plates. After 4 days in culture, hESCs were stained with OCT4, a marker for hESC pluripotency (green) and nuclei (Hoechst, blue). Red lines are drawn to a larger image from two of the wells, showing more hESC OCT4-positive (upper) and a low or average number of hESC OCT4-positive cells in control wells (lower). (B): A representative heat map from a Biomol Enzyme plate showing targets that have increased numbers of OCT4-positive hESCs. Red and black cells represent increased hESC numbers relative to controls and green cells represent decreased or unchanged numbers of hESCs. (C): Cluster analysis was used to identify positive hits that improve hESC growth based on W2 (number of OCT4-positive cells or percentage of W2 compared with control wells; Materials and Methods). Abbreviation: hESC, human embryonic stem cell.