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. 2014 Mar 21;9(3):e88950. doi: 10.1371/journal.pone.0088950

Figure 6. A FRA1-dependent autocrine TGFβ2 loop promotes mesenchymal gene expression in BE CRC cells.

Figure 6

(A and B) Expression of selected mesenchymal (TGFBI, AXL) and epithelial (CDH1, CLDN7) FRA1EMT genes upon transient knockdown of the TGFβ pathway FRA1 targets TGFB2 and SMAD3 using siRNA pools in BE cells. Data are represented relative to levels of these genes in cells transfected with siRNAs targeting GFP. (C) Effects of the TGFβ receptor inhibitor SB43152 (10 µM for 72 h) on expression of a selected mesenchymal FRA1EMT (TGFBI, AXL) genes in BE cells. Student's t-test was used for all comparisons (*p<0.05, **p<0.01, ***p<0.001). Error bars represent S.E.M. for 3 independent experiments.