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. 2014 Mar 21;9(3):e90467. doi: 10.1371/journal.pone.0090467

Figure 2. Tobacco dependence treatment medications (varenicline, bupropion) alter the larval zebrafish nicotine response.

Figure 2

(A–C) Larvae pretreated in varenicline (50 μM) overnight and challenged with stimulus at 6 dpf (± SE). Varenicline attenuates (A) 20 μM nicotine response, but not (B) 50 μM cinnamon oil or (C) 25 μM mustard oil response. (D) Acute treatment with 50 μM varenicline does not affect locomotion at 5 dpf (± SE). (E–F) Mean cumulative distance traveled in the 4 minutes post stimulus exposure as a percent of the average untreated stimulus response (± SE). Wash Nic = 24-hour washout period following acute nicotine experiment and re-tested at 7 dpf. Acute early and acute late response represents the first 4 minutes and last 4 minutes, respectively, post drug exposure at 5 dpf. (E) Movement quantitation for varenicline experiments. (F) Movement quantitation for bupropion experiments. n≥30 larvae per condition; *  = p<0.05; Students t-test.