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. 2014 Mar 21;9(3):e91867. doi: 10.1371/journal.pone.0091867

Figure 4. hBD-1 overexpression showed no significant effect on proliferation of HSC-3, UM1, SCC-9 and SCC-25 cells in vitro.

Figure 4

A) OSCC cells transfected with pGCHBD-1 plasmid showed similar viability compared with control cells in a span of 96 hours. B) Treatment with recombinant hBD-1 protein (50 µg/ml) did not have any significant effect on the viability of HSC-3, UM1, SCC-9 or SCC-25 cells over a period of 48 hours. C) Exogenous expression of hBD-1 did not have any significant effect on colony formation ability of these two cell lines, either (left panel: crystal violet staining; right panel, relative colony number). D) Flow cytometry analysis of cell apoptosis by Annexin-V FITC and PI double staining after transfection with pGCHBD-1 or control plasmid.