Figure 1. Characterization of granulocytic bone marrow cells of transgenic HNP-1 mice deficient in C/EBP-ε.

Murine bone marrow cells were extracted from wild type and transgenic HNP-1 mice with or without deficiency in C/EBP-ε. Erythrocytes and non-granulocytic cells removed by ammonium chloride-based lysis and by immunomagnetic sorting, respectively. Comparative quantification of mRNA for CCAAT/enhancer binding protein-ε (Cebpe), human neutrophil peptide-1 (DEFA1), myeloperoxidase (Mpo), 24p3 (Lcn2), lactoferrin (Ltf), cathelicidin antimicrobial peptide (Camp), matrix metalloproteinase-9 (Mmp9) was done by real-time PCR using Gapdh as normalizer. Figure depicts expression levels relative to HNP-1, Cebpe^+/+ mice. Error bars were calculated by Stratagene MxPro 4.1. Data are representative of two independent experiments.