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. 2014 Mar 21;9(3):e92471. doi: 10.1371/journal.pone.0092471

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© 2014 Glenthøj et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Murine bone marrow from wild type and transgenic HNP-1 mice with or without deficiency in C/EBP-ε was extracted. Non-granulocytic cells were removed by ammonium chloride-based lysis and by immunomagnetic sorting. Cells were spun onto slides, fixed, permeabilized, and stained: (A-D) May-Grünwald Giemsa, (E-H) HNP, (I-L) proHNP, and (M-P) IgG control. Bars represent 20 μm.