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. 2014 Mar 21;9(3):e92438. doi: 10.1371/journal.pone.0092438

Figure 1. Phylogenetic tree of H. meleagridis isolates based on partial 18S rRNA sequences (approx. 544bp).

Figure 1

For the analysis 47 sequences (sequence types) were used. The phylogenetic analysis was performed by applying separately maximum likelihood and neighbor-joining methods, with Trichonympha agilis as outgroup. The tree generated by maximum likelihood method is shown. Samples (N = 34) originating from this study are labelled bold. The robustness of the tree was determined by bootstrap re-sampling of the multiple–sequence alignments (1000 sets neighbor-joining/100 sets maximum likelihood). Values on the nodes are bootstrap support values indicated as percentages from neighbor-joining and maximum likelihood, respectively. Asterisks indicate nodes with bootstrap values below 50% or with a different topology. Branch lengths are proportional to sequence divergence and can be measured relative to the scale bar shown (bottom right). The scale represents nucleotide substitution per position.