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. 2014 Mar 21;9(3):e92438. doi: 10.1371/journal.pone.0092438

Figure 5. Analysis of ITS1-5.8S rRNA sequences from different H. meleagridis clonal cultures demonstrates the presence of multiple sequence variants within a single clone.

Figure 5

(A) Phylogenetic tree based on the complete ITS1-5.8S rRNA-ITS2 region. Phylogenetic analysis of ITS1-5.8S rRNA-ITS2 region was performed using MegAlign application of Lasergene software (DNASTAR Inc.) by applying distance methods with default settings. Different clones are labelled with c and number, e.g. c1. (B) Sequence alignment of 5.8S rDNA region. Only sequence differences are shown, conserved nucleotides are designated as dots. Sequences originating from the same clonal culture are separated with lines.