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. 2014 Mar 21;9(3):e92438. doi: 10.1371/journal.pone.0092438

Table 2. Primers used in this study.

primer name Sequence 5′ to 3′ origin
HmRpb1-F2 GTN ATH TTY AAY CGD CAR CC this study*
HmRpb1-R2 CTT CVA TGT TYT GYT GAC C this study*
HmRpb1-F3 TTG CTA CTG TAG GTC AAC A this study
HmRpb1-R3 ATA ACT GCA TGA TGT CCC ATC A this study
Rpb1-F1 GAG TGT CCA GGN CAY TTY GG [20]
Rpb1_mod_R2 AAT TGT GTA GMT GGT TCA CC this study*
HmRpb1-longF ATT GCG TAT GCC ACA AAT G this study
HmRpb1-longR ATA GCA CCT ACC ATT TCA C this study
Hm-Rpb1-shortF GGA TCA CTT ACA CGA CAA GAC this study
HmRpb1-shortR TCG TTG TTC GAC TTC TTG C this study
HmRpb1-short2F AGA TCC AGA ACG ATC TCA TCC- this study
HmRpb1-short2R TGA CTT AAG AAA TCT CGT GCC this study
18S-F GCA GTT AAA ACG CTC GTA GTC-3’ this study
18S-R AAC GCT AGA CAG GTC AAC CC this study
actinin1-CH-EFhF GCA AAA CAC CTT GCC ACT AAG this study
actinin1-CH-EFhR GCA CGC TTC TCT TCA AGT TC this study
TFR2 CGG TAG GTG AAC CTG CCG TTG G [13]
TFR1 TGC TTC AGT TCA GCG GGT CTT CC [13]

* design based °n the Rpb1 alignment of different parabasalid sequences.