Table 2. Primers used in this study.
primer name | Sequence 5′ to 3′ | origin |
HmRpb1-F2 | GTN ATH TTY AAY CGD CAR CC | this study* |
HmRpb1-R2 | CTT CVA TGT TYT GYT GAC C | this study* |
HmRpb1-F3 | TTG CTA CTG TAG GTC AAC A | this study |
HmRpb1-R3 | ATA ACT GCA TGA TGT CCC ATC A | this study |
Rpb1-F1 | GAG TGT CCA GGN CAY TTY GG | [20] |
Rpb1_mod_R2 | AAT TGT GTA GMT GGT TCA CC | this study* |
HmRpb1-longF | ATT GCG TAT GCC ACA AAT G | this study |
HmRpb1-longR | ATA GCA CCT ACC ATT TCA C | this study |
Hm-Rpb1-shortF | GGA TCA CTT ACA CGA CAA GAC | this study |
HmRpb1-shortR | TCG TTG TTC GAC TTC TTG C | this study |
HmRpb1-short2F | AGA TCC AGA ACG ATC TCA TCC- | this study |
HmRpb1-short2R | TGA CTT AAG AAA TCT CGT GCC | this study |
18S-F | GCA GTT AAA ACG CTC GTA GTC-3’ | this study |
18S-R | AAC GCT AGA CAG GTC AAC CC | this study |
actinin1-CH-EFhF | GCA AAA CAC CTT GCC ACT AAG | this study |
actinin1-CH-EFhR | GCA CGC TTC TCT TCA AGT TC | this study |
TFR2 | CGG TAG GTG AAC CTG CCG TTG G | [13] |
TFR1 | TGC TTC AGT TCA GCG GGT CTT CC | [13] |
* design based °n the Rpb1 alignment of different parabasalid sequences.