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. 2014 Mar 21;9(3):e92564. doi: 10.1371/journal.pone.0092564

Figure 1. Ex vivo production of tumor necrosis factor alpha (TNF-α) by human neutrophils.

Figure 1

The release of TNF-α from non-stimulated (control, C) neutrophils, or LPS- (LPS), Pam3Csk4- (Pam), and fMLP- (fMLP) stimulated neutrophils after 90 minutes were measured with enzyme-linked immunosorbent assay. Boxes represent median (interquartile range). Kruskal-Wallis test with post-hoc analysis by Mann-Whitney test using Bonferroni's corrections were used to compare responses with and without PRRs ligands. *p<0.05. These data are representative of two different experiments.