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. 2014 Mar 21;9(3):e92495. doi: 10.1371/journal.pone.0092495

Figure 1. Anti-ΔC NS1 and anti-DJ NS1 Abs show lower binding activity to human endothelial cells and platelets than anti-full-length DENV NS1 Abs.

Figure 1

(A) The C-terminal region of DENV NS1 protein from a.a. 271-352 was deleted to generate ΔC NS1 protein. The DJ NS1 protein consists of the N-terminus of DENV NS1 (a.a. 1-270) and the C-terminus of JEV NS1 (a.a. 271-352). Polyclonal Abs against DENV NS1, ΔC NS1, or DJ NS1 were generated in mice and purified on protein G columns. Formaldehyde-fixed human endothelial cells (B) and platelets (C) were incubated with control IgG, anti-DENV NS1, anti-ΔC NS1 or anti-DJ NS1 Abs, followed by Alexa 488-conjucated anti-mouse IgG and analyzed by flow cytometry. The averages of triplicate cultures are shown. * P<0.05, ** P<0.01, *** P<0.001.