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. 2014 Mar 21;9(3):e92495. doi: 10.1371/journal.pone.0092495

Figure 5. Anti-DENV NS1, anti-ΔC NS1 and anti-DJ NS1 Abs reduce DENV NS3 expression.

Figure 5

(A) The local skin was collected and fixed in paraffin. The skin sections were stained with anti-DENV NS3 Abs, followed by HRP-conjugated anti-rabbit IgG. Nuclei were stained with hematoxylin (blue). The arrows indicate positive staining. (a)–(e) show the NS3 expression of cells in the dermis layer of skin sections. (Magnification: ×200; bar = 100 μm) (B) Quantification of NS3 staining was performed on skin sections using HistoQuest analysis software. A representative scattergram plot of each group is shown and the percentage of NS3-positive cells is shown as means ± SD obtained from three mice of each group. *** P<0.001 as compared with medium control group. # P<0.05; ## P<0.01 as compared with DENV plus control IgG group. (C) Skin cryosections were stained with anti-DENV NS3 and CD31 Abs, followed by Alexa-488-conjugated anti-rabbit IgG and Alexa-594-conjugated anti-rat IgG. Nuclei were stained with DAPI. (Magnification: ×200; bar = 50 μm)