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. 2014 Mar 21;9(3):e92495. doi: 10.1371/journal.pone.0092495

Figure 6. Anti-DENV NS1, anti-ΔC NS1 and anti-DJ NS1 Abs cause complement-mediated cytolysis and inhibit virus release into the culture medium.

Figure 6

(A) HMEC-1 cells were infected with DENV (MOI = 10) for 72 h. At 72 h post-infection, cells were incubated with control IgG, anti-DENV NS1, anti-ΔC NS1 or anti-DJ NS1 Abs (50 μg/ml) for 1 h at 4°C and incubated with or without complement for 4 h at 37°C. Cell culture supernatants were collected and detected for the release of lactate dehydrogenase (LDH). * P<0.05; ** P<0.01. (B) At 6 h post-infection, DENV-infected HMEC-1 cells were incubated with control IgG, anti-DENV NS1, anti-ΔC NS1 or anti-DJ NS1 Abs (50 μg/ml) in the presence of complement (C′) and cultured at 37°C. Virus titers in the culture medium at the indicated time points were measured by plaque assay. The averages of triplicate cultures are shown. * P<0.05; ** P<0.01; *** P<0.001 as compared with C′ alone group. # P<0.05; ## P<0.01; ### P = 0.076 as compared with C′ plus control IgG group.