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. 2014 Mar 21;9(3):e92377. doi: 10.1371/journal.pone.0092377

Figure 1. Deletion mutant Seg-10 used in reverse genetics for virus rescue.

Figure 1

Deletions were made throughout the ORF of Seg-10. Mutant ΔA was not viable as indicated by a cross. Protein domains encoded by Seg-10 are indicated using different colours. BD = binding domain, LD = late domain, IC = intracellular, TM = trans membrane, EC = extracellular. Nucleotide positions are indicated with numbers. Segment length is indicated next to the illustrations. (A) Mutant segments with consecutive deletions throughout the original Seg-10. (B) Mutants based on segment ΔD, but with inserted viral sequences. Insertions of Seg-1 and Seg-2 are shown in purple and pink, respectively. The orientation of insertions are indicated by arrows. (C) ΔD(S2) segments, but with the insertion in a different location or orientation or with an additional deletion or with the GFP sequence(bright green) inserted.