Skip to main content
. 2014 Mar 21;9(3):e92377. doi: 10.1371/journal.pone.0092377

Figure 5. dsRNA of Seg-10 deletion mutant viruses.

Figure 5

dsRNA was isolated from cells infected with passage 4 of all Seg-10 deletion mutant viruses. Black dots indicate the segments 1–10 of BTV1, with Seg-5 and Seg-6 almost at the same position in the gel. A black dot also indicates the band with the expected size of Seg-10 based on the deletion, for the different mutant viruses. White dots indicate Seg-10 bands of mutant viruses, different from deletion Seg-10 of the expected size. All mutant viruses contain a band with the size of the original deletion Seg-10. All Seg-10 deletion mutant viruses contain Seg-10 variants, except for ΔB and ΔH. Note that the ladder used is made of dsDNA, so the height in the gel of the dsRNA cannot be used to determine the exact size of the band.