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(A) pCAG-Myc-mLin-7A or (B) pCAG-Myc-mLin-7B was co-transfected into COS7 cells with control pSUPER vector, pSUPER–mLin7A#1, or pSUPER–mLin7A#2. After 48 h, cells were harvested and subjected to western blotting (20 μg protein per lane) with anti-Myc. Anti-β-tubulin was used as a loading control. (C), pCAG-Myc-hLin-7A was co-transfected into COS7 cells with control pSUPER vector or pSUPER–mLin7A#1. Analyses were done as in A.
