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. 2014 Mar 21;9(3):e92641. doi: 10.1371/journal.pone.0092641

Figure 1. FOP-FGFR1V74F/E97K mutant lacks centrosome localization but not kinase activity.

Figure 1

(A) Schematic of fusion between FOP and FGFR1 resulting in the FOP-FGFR1 oncogenic fusion and subsequent point mutations to produce KD, FOP-FGFR1K259A, and centrosome localization mutant, FOP-FGFR1V74F/E97K. (B) WB analysis of lysates from RPE-1 cells transfected with WT Myc-FOP-FGFR1, Myc-FOP-FGFR1K259A, or Myc-FOP-FGFR1V74F/E97K, harvested, and probed with antibodies against phosphotyrosine (PY), Myc, and p38 as a loading control. (C) RPE-1 cells transfected with Myc-FOP-FGFR1, Myc-FOP-FGFR1K259A, or Myc-FOP-FGFR1V74F/E97K, fixed, and stained with antibodies against Myc (green) and PY (red). DNA is stained using DAPI (blue). Scale bars: 10 μm; insets: 10× magnification.