Table 2. Oligonucleotides.
Names | Genes | Sequences |
KCP021 | ompA-Fm | ATAAGTACCGCATAAAACCTACTATTGCTCCGCGTTTTTTACTGTAGGCTGGAGCTGCTTCG |
KCP022 | ompA-Rm | GGGGCGTCGTCGCCCCAAAAAGATGGTCTGCTCTTGAATTCGCATATGAATATCCTCCTTAGTTCC |
KCP023 | ecpA-Fm | CCCTGTAGTGCAGGAGTTAAGTTGAGCCCTTCTTTATGTTACTGTAGGCTGGAGCTGCTTCG |
KCP024 | ecpA-Rm | TCGACCCCCCGATGGGGACGACCATGTAGTCTCTCTAATTGACATATGAATATCCTCCTTAGTTCC |
KCP025 | fimA-Fm | CATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGTGTAGGCTGGAGCTGCTTCG |
KCP026 | fimA-Rm | GTCGCATCCGCATTAGCAGCACCCGGGGTTGCCTCGCCCATATGAATATCCTCCTTAGTTCC |
KCP027 | pgaA-Fm | ATGTCTCTCTCTAAAACCGTTATGTACCTCATTATGTCCTACTGTAGGCTGGAGCTGCTTCG |
KCP028 | pgaA-Rm | CCTCTATAAATAAAGGTAATGCATTGTATAAATAGGAATTTTCATATGAATATCCTCCTTAGTTCC |
KCP029 | nanA-Fm | TAGTCTGTTCGTAGTGAAGTCTCCATAAATACCGTTGCTTAATGTAGGCTGGAGCTGCTTCG |
KCP030 | nanA-Rm | GGGGAGTGGGCCATCCCCGCTCGCTCCCCTTTGTTGAGTGGGCATATGAATATCCTCCTTAGTTCC |
KCP031 | csgA-Fm | TTCCATTCGACTTTTAAATCAATCCGATGGGGGTTTTACATGTGTAGGCTGGAGCTGCTTCG |
KCP032 | csgA-Rm | GGGCTTGCGCCCTGTTTCTTTAATACAGATGATGTATTAGTACATATGAATATCCTCCTTAGTTCC |
KCP063 | lpfA-Fm | TAAAATTATGTAGTTCTAAAAGAAAAATTACATTAAAAAATTTGTAGGCTGGAGCTGCTTCG |
KCP064 | lpfA-Rm | CTGATTACCGCCGTTAATGCGGCGGTAAACATTTTGCCTGCTCATATGAATATCCTCCTTAGTTCC |
KCP065 | eae-Fm | ATTAAATAAAGAGTAAGATTGAGTAACACCACCTCGGTATTGTGTAGGCTGGAGCTGCTTCG |
KCP066 | eae-Rm | GGCTGATTTTGTTATGTATAAAATCGGCCCCACCAATACCTTCATATGAATATCCTCCTTAGTTCC |
KCP067 | tir-Fm | TTCCTAACAATAGATAAATGTATTTTATTTTTCCTCTATAAATGTAGGCTGGAGCTGCTTCG |
KCP068 | tir-Rm | AAGGGGGGAGGGAGGGAGATTTATTTTACTAATACCTATATACATATGAATATCCTCCTTAGTTCC |
KCP069 | espA-Fm | TAAAAAAACAAAAGGACTCTTTTTAATAGTTCTCCATATATCTGTAGGCTGGAGCTGCTTCG |
KCP070 | espA-Rm | TCTGTCCCATAGCAATAAATGCAATTCGTATCAATAGAGGCCCATATGAATATCCTCCTTAGTTCC |
KCP071 | fliA-Fm | CTATTAGTACGGCTATTGAGTATATTGCGTCCCGACAAATAGTGTAGGCTGGAGCTGCTTCG |
KCP072 | fliA-Rm | CTTGAGGACCATCAGTTTCAATTTCACGCCGTAAATGACTGCCATATGAATATCCTCCTTAGTTCC |
KCP073 | yehD-Fm | TCTGAGAATTGTTTTGTTTTATTTGAATAATTCCTTACGTAGTGTAGGCTGGAGCTGCTTCG |
KCP074 | yehD-Rm | GTAAAATTGAATGACTTTTTTGTTCTACTAATAAAATTTATACATATGAATATCCTCCTTAGTTCC |
KCP075 | yraH-Fm | ATTAAATATGATTATGTACTTGTTACAAGGATAAGGTTATAATGTAGGCTGGAGCTGCTTCG |
KCP076 | yraH-Rm | GAAAAGGGCGTTATCTGAAAGGTCAGATAACGCCGTAACGTACATATGAATATCCTCCTTAGTTCC |
KCP077 | sfmA-Fm | TACTATCAGTGTCTTAAATAAAGTAATCGGTTATATACGGATTGTAGGCTGGAGCTGCTTCG |
KCP078 | sfmA-Rm | GTTATAAGACGTGATATATAATTCAAAACAACGTGGTTTTGACATATGAATATCCTCCTTAGTTCC |
KCP079 | yfcV-Fm | GTTACAAATATAAAAATTAATAAATACCAAATTCCTGTTTATTGTAGGCTGGAGCTGCTTCG |
KCP080 | yfcV-Rm | GGTAAATTTTTGGGGTATCGGGCTTTCGATACCCCCATAGTGCATATGAATATCCTCCTTAGTTCC |
KCP708 | ompA-F | ATGATAACGAGTCGACAAAAATGAAAAAGACAGCTATCGCGATTG |
KCP709 | ompA-R | CCCGGAATTCTTAAGCTTGCGGCTGAGTTACAAC |
Primers were designed for gene inactivation in chromosomal DNA of E. coli O157:H7.