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. 2014 Mar 21;9(3):e92723. doi: 10.1371/journal.pone.0092723

Table 2. Oligonucleotides.

Names Genes Sequences
KCP021 ompA-Fm ATAAGTACCGCATAAAACCTACTATTGCTCCGCGTTTTTTACTGTAGGCTGGAGCTGCTTCG
KCP022 ompA-Rm GGGGCGTCGTCGCCCCAAAAAGATGGTCTGCTCTTGAATTCGCATATGAATATCCTCCTTAGTTCC
KCP023 ecpA-Fm CCCTGTAGTGCAGGAGTTAAGTTGAGCCCTTCTTTATGTTACTGTAGGCTGGAGCTGCTTCG
KCP024 ecpA-Rm TCGACCCCCCGATGGGGACGACCATGTAGTCTCTCTAATTGACATATGAATATCCTCCTTAGTTCC
KCP025 fimA-Fm CATGTCGATTTAGAAATAGTTTTTTGAAAGGAAAGCAGCATGTGTAGGCTGGAGCTGCTTCG
KCP026 fimA-Rm GTCGCATCCGCATTAGCAGCACCCGGGGTTGCCTCGCCCATATGAATATCCTCCTTAGTTCC
KCP027 pgaA-Fm ATGTCTCTCTCTAAAACCGTTATGTACCTCATTATGTCCTACTGTAGGCTGGAGCTGCTTCG
KCP028 pgaA-Rm CCTCTATAAATAAAGGTAATGCATTGTATAAATAGGAATTTTCATATGAATATCCTCCTTAGTTCC
KCP029 nanA-Fm TAGTCTGTTCGTAGTGAAGTCTCCATAAATACCGTTGCTTAATGTAGGCTGGAGCTGCTTCG
KCP030 nanA-Rm GGGGAGTGGGCCATCCCCGCTCGCTCCCCTTTGTTGAGTGGGCATATGAATATCCTCCTTAGTTCC
KCP031 csgA-Fm TTCCATTCGACTTTTAAATCAATCCGATGGGGGTTTTACATGTGTAGGCTGGAGCTGCTTCG
KCP032 csgA-Rm GGGCTTGCGCCCTGTTTCTTTAATACAGATGATGTATTAGTACATATGAATATCCTCCTTAGTTCC
KCP063 lpfA-Fm TAAAATTATGTAGTTCTAAAAGAAAAATTACATTAAAAAATTTGTAGGCTGGAGCTGCTTCG
KCP064 lpfA-Rm CTGATTACCGCCGTTAATGCGGCGGTAAACATTTTGCCTGCTCATATGAATATCCTCCTTAGTTCC
KCP065 eae-Fm ATTAAATAAAGAGTAAGATTGAGTAACACCACCTCGGTATTGTGTAGGCTGGAGCTGCTTCG
KCP066 eae-Rm GGCTGATTTTGTTATGTATAAAATCGGCCCCACCAATACCTTCATATGAATATCCTCCTTAGTTCC
KCP067 tir-Fm TTCCTAACAATAGATAAATGTATTTTATTTTTCCTCTATAAATGTAGGCTGGAGCTGCTTCG
KCP068 tir-Rm AAGGGGGGAGGGAGGGAGATTTATTTTACTAATACCTATATACATATGAATATCCTCCTTAGTTCC
KCP069 espA-Fm TAAAAAAACAAAAGGACTCTTTTTAATAGTTCTCCATATATCTGTAGGCTGGAGCTGCTTCG
KCP070 espA-Rm TCTGTCCCATAGCAATAAATGCAATTCGTATCAATAGAGGCCCATATGAATATCCTCCTTAGTTCC
KCP071 fliA-Fm CTATTAGTACGGCTATTGAGTATATTGCGTCCCGACAAATAGTGTAGGCTGGAGCTGCTTCG
KCP072 fliA-Rm CTTGAGGACCATCAGTTTCAATTTCACGCCGTAAATGACTGCCATATGAATATCCTCCTTAGTTCC
KCP073 yehD-Fm TCTGAGAATTGTTTTGTTTTATTTGAATAATTCCTTACGTAGTGTAGGCTGGAGCTGCTTCG
KCP074 yehD-Rm GTAAAATTGAATGACTTTTTTGTTCTACTAATAAAATTTATACATATGAATATCCTCCTTAGTTCC
KCP075 yraH-Fm ATTAAATATGATTATGTACTTGTTACAAGGATAAGGTTATAATGTAGGCTGGAGCTGCTTCG
KCP076 yraH-Rm GAAAAGGGCGTTATCTGAAAGGTCAGATAACGCCGTAACGTACATATGAATATCCTCCTTAGTTCC
KCP077 sfmA-Fm TACTATCAGTGTCTTAAATAAAGTAATCGGTTATATACGGATTGTAGGCTGGAGCTGCTTCG
KCP078 sfmA-Rm GTTATAAGACGTGATATATAATTCAAAACAACGTGGTTTTGACATATGAATATCCTCCTTAGTTCC
KCP079 yfcV-Fm GTTACAAATATAAAAATTAATAAATACCAAATTCCTGTTTATTGTAGGCTGGAGCTGCTTCG
KCP080 yfcV-Rm GGTAAATTTTTGGGGTATCGGGCTTTCGATACCCCCATAGTGCATATGAATATCCTCCTTAGTTCC
KCP708 ompA-F ATGATAACGAGTCGACAAAAATGAAAAAGACAGCTATCGCGATTG
KCP709 ompA-R CCCGGAATTCTTAAGCTTGCGGCTGAGTTACAAC
m

Primers were designed for gene inactivation in chromosomal DNA of E. coli O157:H7.