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. 2014 Mar 21;9(3):e92920. doi: 10.1371/journal.pone.0092920

Figure 4. BapR and QS are required for maximal expression of bapA and for maximal biofilm formation.

Figure 4

A, a mutant cepI rpfFBc, Prha-bapR and harboring a PbapA-lacZ promoter fusion was grown in the presence of rhamnose, C8-AHL, BDSF or the combination of these molecules. Expression of bapA was determined measuring the activity of the reporter gene. B, biofilm formation was quantified in a mutant cepI rpfFBc, Prha-bapR after the addition of rhamnose, C8-AHL, BDSF or the combination of these molecules. Error bars, SEM. Asterisks denote significant differences in bapA expression or in biofilm formation between the samples after supplementing with rhamnose, C8-AHL, BDSF or the combination of these molecules (1-way ANOVA, *** p<0.0001, n≥3).