Table 2. Non-homologous end-joining junction sequences.
| Sce.white Sequence | |||
|---|---|---|---|
| GAGCTGTTTGAGCTGTAGGGATAA |
CAGGGTAATAGCTCTTTGACA |
||
| Isolate No. | Junction Sequence | Δ bp | |
| 15 | GAGCTGTTTGAGCTGTAGGGATAA | -—————–TAGCTCTTTGACA | −8 |
| 19 | GAGCTGTTTGAGCTGTAGGGATAA | -—————–TAGCTCTTTGACA | −8 |
| 21 | GAGCTGTTTGAGCTGTAGGGATAA | -—————–TAGCTCTTTGACA | −8 |
| 7 | GAGCTGTTTGAGCTGTAGGGATAA (TAA) | CAGGGTAATAGCTCTTTGACA | +3 |
| 13 | GAGCTGTTTGAGCTGTAGGG——–- | CAGGGTAATAGCTCTTTGACA | −4 |
| 22 | GAGCTGTTTGAGCTGTA-GGATAA | CAGGGTAATAGCTCTTTGACA | −1 |
| 40 | GAGCTGTTTGAGCT——————––- | CAGGGTAATAGCTCTTTGACA | −10 |
| 1(mu) | GAGCTGTTTGAGCTGTAGGGATAA | -—————–TAGCTCTTTGACA | −8 |
| 11(mu) | GAGCTGTTTGAGCTGTAGGG——–- | CAGGGTAATAGCTCTTTGACA | −4 |
| 22(mu) | GAGCTGTTTGAGCTGTAGGGATAA | -—————–TAGCTCTTTGACA | −8 |
| 25(mu) | GAGCTGTTTGAGCTGTAG(T)GGATAA | CAGGGTAATAGCTCTTTGACA | +1 |
| 27(mu) | TTCCCGCTTACACACAATTGCACAa | CAGGGTAATAGCTCTTTGACA | −780, +818 |
Sequence inserted at wild-type SacI site is indicated in bold, creating Sce.white. The 18-bp I-SceI recognition sequence is italicized. Sequence of isolated nonhomologous end-joining junction events are shown. Microhomologies are underlined, and changes in bp number are indicated as either deletion (−) or insertion (+).
a
27(mu) included a deletion of 780 bp from Sce.white (upstream of DSB) and 818-bp insertion of y+ in the opposite orientation.