Table 3. Frequency of each class of progeny from vials separated by individual or by time.

Y Chromosome			X		12		X1		2		X2		1
1	2		Average	SD	Average	SD	Average	SD	Average	SD	Average	SD	Average	SD
ROMA	10B
		123	0.123	0.020	0.092	0.028	0.225	0.003	0.203	0.040	0.182	0.010	0.223	0.020
		1	0.145	0.058	0.070	0.016	0.230	0.026	0.126	0.210	0.183	0.033	0.231	0.105
		2	0.119	0.012	0.128	0.039	0.226	0.042	0.149	0.112	0.166	0.058	0.205	0.056
		3	0.114	0.037	0.088	0.049	0.227	0.073	0.183	0.087	0.189	0.012	0.226	0.044
		0.1	0.103	0.019	0.065	0.026	0.256	0.051	0.147	0.116	0.218	0.015	0.205	0.058
		0.2	0.140	0.062	0.105	0.028	0.202	0.035	0.150	0.126	0.159	0.034	0.213	0.063
		0.3	0.135	0.018	0.116	0.056	0.225	0.043	0.155	0.179	0.161	0.016	0.245	0.090
ROMA	473
		123	0.117	0.017	0.187	0.016	0.150	0.024	0.191	0.036	0.249	0.048	0.105	0.013
		1	0.140	0.048	0.164	0.064	0.158	0.010	0.236	0.042	0.188	0.059	0.113	0.044
		2	0.110	0.061	0.192	0.090	0.122	0.053	0.182	0.023	0.281	0.065	0.113	0.034
		3	0.112	0.059	0.190	0.050	0.169	0.056	0.157	0.026	0.282	0.078	0.089	0.022
		0.1	0.127	0.045	0.167	0.045	0.187	0.042	0.174	0.049	0.241	0.049	0.104	0.033
		0.2	0.095	0.046	0.216	0.068	0.137	0.029	0.194	0.007	0.246	0.070	0.112	0.019
		0.3	0.141	0.066	0.162	0.077	0.126	0.046	0.208	0.068	0.265	0.121	0.098	0.050

Two chromosome combinations were tested, X/Y, ROMA, Y, rDNA^wt-10B, and X/Y, ROMA/Y, rDNA^l-473. Each of three replicate vials (“1,” “2,” and “3”) from each genotype were transferred twice, establishing three temporal replicates (“0.1,” “0.2,” and “0.3”) from each. For example, 1.1, 1.2, and 1.3 were established by the same individuals, each offset by 5 days, whereas 1.1, 2.1, and 3.1 were all set on the same day with a separate set of parents. Progeny were scored independently and all nine of a genotype were considered as a set (“123”) or analyzed as progeny of parents or as progeny from a set time. In only one case do 0.975 confidence intervals of every pairwise comparison exceed 0 (bold), showing that within Bayesian limits there is no difference between progeny frequencies from any two vials, indicating that the variance seen is not attributable to differences in heritable or temporal factors.