Figure 3.

Expression of cell-cycle transcription regulators (A) and cell-cycle target genes (B, C) in ess1^H164R mutants. Total RNA was harvested from wild-type and ess1^H164R cells, reverse transcribed, and quantitated with the use of reverse-transcription polymerase chain reaction (see the Materials and Methods). Data are presented as fold-change in expression of the indicated gene in ess1^H164R mutant cells relative to the wild type. Three biological replicates were used for each strain and error bars are the SD from the mean. For samples in (C), cells were grown in yeast extract peptone dextrose at 30°, arrested for 3.0 hr with 15 μg/mL nocodazole, released, and grown for the indicated times prior to harvesting for RNA analysis.