Table 4. Segregation analysis of double mutants.
| Cross (Relevant Genotypes) | Total Tetrads | No. Viable Spores | ||||
|---|---|---|---|---|---|---|
| 4 | 3 | 2 | 1 | 0 | ||
| ESS1 x swi4Δ | 22 | 5 | 8 | 7 | 2 | 0 |
| ess1H164R x swi4Δ | 23 | 11 | 8 | 4 | 0 | 0 |
| ESS1 x swi6Δ | 31 | 15 | 14 | 2 | 0 | 0 |
| ess1H164R x swi6Δ | 34 | 3 | 20 | 4 | 6 | 1 |
| ESS1 x whi3Δ | 24 | 17 | 5 | 2 | 0 | 0 |
| ess1H164R x whi3Δ | 24 | 16 | 6 | 1 | 1 | 0 |
| ESS1 x whi5Δ | 21 | 14 | 2 | 5 | 0 | 0 |
| ess1H164R x whi5Δ | 24 | 20 | 3 | 1 | 0 | 0 |
| ESS1 x mbp1Δ | 23 | 5 | 16 | 2 | 0 | 0 |
| ess1H164R x mbp1Δ | 23 | 10 | 11 | 2 | 0 | 0 |
Strains used in crosses: ESS1 = YDA579; ess1H164R = YDA582; deletion mutants are from EUROSCARF collection. Frequency in which the dead spore is a double mutant (deduced) in tetrads with 3:1 segregation for growth: ess1H164R swi4Δ = 6/8; ess1H164R swi6Δ = 16/20; ess1H164R whi3Δ = 2/6; ess1H164R whi5Δ = 0/3; and ess1H164R mbp1Δ = 3/11.