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. 2014 Mar 21;9(3):e92949. doi: 10.1371/journal.pone.0092949

Figure 1. PKM2 interacts with HSP40/DNAJB1 in vivo and in vitro.

Figure 1

(A) Growth of yeast expressing HSP40/DNJB1-GalAD and PKM2-GalBD on nutritional deficient SD medium (-Trp-Leu or -Trp-Leu-Ade-His). Vector pGADT7-T and pGBKT7-p53 performed as positive control, pGADT7-T and pGBKT7-Lam performed as negative control. (B) Cell lysates of HEK293T cells transfected with HA-PKM2 and Flag-HSP40 were immunoprecipitated with anti-HA antibody, the bound proteins were detected by anti-Flag antibodies. (C) Cell lysates of HEK293T cells transfected with HA-PKM2 and Flag-HSP40 were immunoprecipitated with anti-Flag antibody, the bound proteins were detected by anti-HA antibodies. (D) HeLa cells were lysed and Co-IP was performed with anti-PKM2 antibody, then western blot was analyzed using anti-PKM2 and anti-HSP40 antibodies. (E) HeLa cells were lysed and Co-IP was performed with anti-HSP40 antibody, then western blot was analyzed using anti-PKM2 and anti-HSP40 antibodies. (F) Immunofluorescence analysis reveals that HSP40 and PKM2 co-localize with each other in nucleus in HeLa cells.