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. Author manuscript; available in PMC: 2015 Mar 15.
Published in final edited form as: Cancer Res. 2014 Feb 3;74(6):1705–1717. doi: 10.1158/0008-5472.CAN-13-1896

Figure 6. UTX-catalyzed H3K27me3 demethylation and MLL4-catalyzed H3K4 methylation occurs in a coordinate manner.

Figure 6

A–C, The effect of UTX knockdown on chromatin levels of MLL4 and H3K4me3 at the proximal promoters of MMP-9 (A), MMP-11 (B), and Six1 (C) in MDA-MB-231 cells. ChIP assays were carried out with anti-MLL4 and anti-H3K4me3 after UTX knockdown. D–F, The effect of MLL4 knockdown on chromatin levels of UTX and H3K27me3 at the proximal promoters of MMP-9 (D), MMP-11 (E), and Six1 (F) in MDA-MB-231 cells. ChIP assays were performed with anti-UTX and anti-H3K27me3 after MLL4 knockdown. Data are presented as the mean ± SEM (error bars) of three independent experiments. *, P<0.05; **, P<0.01; ***, P<0.001.