Transport enhancement of sodium fluorescein and parvalbumin in loaded vs. non-loaded tibial LCS of the 12–13 month-old perlecan-deficient (Hypo) and control (CTL) mice. Two-way ANOVA showed that genotype (F1,56=4.44, P=0.04) and tracer type (F1,56=8.08, P=0.006) had significant effects on the measurements. Although no significant difference was detected for multiple comparison using Bonferroni post hoc tests, the larger parvalbumin moved significantly faster in the Hypo PCM relative to the CTL PCM as assessed by unpaired Student’s t-test (p=0.03). N values of paired FRAP tests per study group ranged from 10–20 lacunae.