Figure 7. H3K79me3 is enriched in differentiating tubular epithelial cells at postnatal day 1. (A) Low power view (×4). Dotted line indicates the renal capsule. (B and C) Low (×10) and high (×40) power views of the renal cortex showing a progressive increase in H3K79me3 in differentiating E-cadherin⁺ tubules. The nephrogenic zone and nascent nephrons (arrowheads) are almost devoid of H3K79me3. (D) Low power view (×10) of the renal cortex in an adult kidney. LTL marks the proximal tubules. H3K79me3 is enriched in both proximal and non-proximal tubules. (E) Co-staining for H3K79me3, carbonic anhydrase-2 (CA2, a marker of intercalated cells), and aquaporin-2 (AQP-2, a marker of principal cells). Magnification ×60. (F) Low power view (×10) of the renal cortex in an adult kidney sections incubated with H3K79me3 antibody alone, H3K79me3 antibody + unmodified H3 N-terminal peptide, or H3K79me3 antibody + H3 K79me3 modified peptide. The specific nuclear staining is absent in the section incubated with the modified peptide.