Table 1.
Target | Catalog No. | Dilution | Temperature, °C | Time, h |
---|---|---|---|---|
CPI-17 | sc-17561 | 1:350 | 4 | O/N |
L-type C1a | sc-16230 | 1:400 | 4 | O/N |
RhoA | sc-418 | 1:400 | RT | 1.5 |
Rock 1 | sc-17794 | 1:150 | 4 | O/N |
Rock 2 | sc-1851 | 1:200 | 4 | O/N |
SERCA2 | sc-8094 | 1:400 | 4 | O/N |
α-Tubulin | MAB1637 | 1:1,000 | 4 | O/N |
All antibodies were purchased from Santa Cruz Biotechnologies, except for α-tubulin (Chemicon). CPI-17, PKC-potentiated phosphatase inhibitor of 17 kDa; Rock, Rho kinase; SERCA2, sarco(endo)plasmic reticulum Ca2+-ATPase 2; RT, room temperature; O/N, overnight.