Skip to main content
. 2014 Jan 30;306(7):G557–G574. doi: 10.1152/ajpgi.00153.2013

Fig. 2.

Fig. 2.

Effect of acidic pH on Dkk1 expression in esophageal cell lines. A: Western blot analysis of baseline Dkk1 protein expression in whole cell lysates of EPC1 and EPC2 cells. β-Actin served as a loading control. B: ELISA of Dkk1 protein secretion in equally seeded EPC1 and EPC2 cells at rest after 24 h of incubation. Values are means ± SE of 3 independent experiments. C and D: quantitative RT-PCR analysis of Dkk1 mRNA expression in EPC1 and EPC2 cells following acute acid exposure (C) and chronic acid pulsing (D). Total RNA was collected 3 h after acid exposure. E: ELISA of Dkk1 protein secretion following chronic acid pulsing. Data represent relative mean Dkk1 secretion. *P ≤ 0.05, **P ≤ 0.01 vs. no acid on the respective day. F: Western blot analysis of Dkk1 protein expression in whole lysates of EPC1 cells following chronic acid pulsing.