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. 2014 Jan 30;306(7):G557–G574. doi: 10.1152/ajpgi.00153.2013

Fig. 9.

Fig. 9.

Wnt/β-catenin signaling activation counteracts acid-induced senescence and is inhibited by Dkk1. A: quantitative analysis of SA-β-Gal staining following chronic acid pulsing in the presence of recombinant human Wnt3a (rhWnt3a) in EPC2 cells. Values are means ± SE of 3 independent experiments. *P ≤ 0.05. B: quantitative RT-PCR analysis of AXIN2 gene expression (a) and TOP/FOP-flash reporter assay (b) in EPC2 cells following 24 h of stimulation with rhDkk1, rhWnt3a, anti-Dkk1 Ab, and human IgG. TOP/FOP-flash activity was normalized to Renilla activity. AXIN2 gene expression was normalized to β-actin expression. TOP/FOP-flash activity values are relative to unstimulated cells of 3 independent experiments. *P ≤ 0.05.